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Figure 4 | Molecular Pain

Figure 4

From: β-arrestin-2-biased agonism of delta opioid receptors sensitizes transient receptor potential vanilloid type 1 (TRPV1) in primary sensory neurons

Figure 4

SNC80 and ARM390 are equally effective at inhibiting N-type voltage-gated calcium currents in primary sensory neurons. TG neurons from rats were nucleofected with rat DOPr-YFP cDNA and N-type voltage-gated calcium currents were recorded 24–48 h after isolation. Maximal current amplitudes were observed between −10 and −5 mV. Control currents were recorded first in SES with 0.1% DMSO (Vehicle). Following bath application of SNC80 (1 μM, 5 min) currents were again recorded. Cells that responded to SNC80 were washed with SES (5 min) and currents were recorded again prior to bath application of ARM390 (1 μM, 5 min) and final current measurement. Representative single cell current–voltage curves (A) and the mean ± SEM extent of current inhibition (expressed as % inhibition) for 10 and 12 cells for SNC80 and ARM390, respectively (B).

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