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Figure 5 | Molecular Pain

Figure 5

From: Cyclic phosphatidic acid relieves osteoarthritis symptoms

Figure 5

Effect of 2ccPA on mRNA expression and production of inflammatory cytokines. To measure mRNA expression, synoviocytes (A) and SW1353 cells (B) were cultured with 1 or 10 μM 2ccPA for 24 h in the presence or absence of 10 ng/mL IL-1β. mRNA levels of each gene were determined by quantitative real-time PCR. Data represent the mean ± SE of triplicate independent experiments (*P < 0.05 and **P < 0.01 vs. vehicle-treated controls). To measure IL-6 concentrations in the culture media of synoviocytes (A) and SW1353 cells (B), cells were cultured with 10 μM 2ccPA, IBF or DCF in the presence of 10 ng/mL IL-1β. After 24 h incubation, culture media were collected, and IL-6 concentrations were determined by ELISA. Data represent the mean ± SE of triplicate independent experiments (*P < 0.05 and **P < 0.01 vs. vehicle-treated controls).

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