Figure 8

Effect of Ki16425 on the suppressive function of 2ccPA on MMP-1, -3, and -13 production in synoviocytes and SW1353 cells. Synoviocytes (A) or SW1353 cells (B) were pre-incubated with 10 μM Ki16425 for 30 min, then treated with 1, 3, or 10 μM 2ccPA for 24 h in the presence of 10 ng/mL IL-1β. The culture media were collected and the concentration of each MMP was measured by ELISA. Data represent the mean ± SE of triplicate independent experiments (*P < 0.05 and **P < 0.01 vs. vehicle-treated controls).