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Figure 4 | Molecular Pain

Figure 4

From: Validation of four reference genes for quantitative mRNA expression studies in a rat model of inflammatory injury

Figure 4

Determination of the optimal number of reference genes by the NormFinder algorithm. Stepwise calculation of stability began with the most stable gene (Mapk6), followed by serial addition of the next most stable gene and calculation of the accumulated standard deviation (circles). In both the dorsal horn (A) and in the RVM (B), the optimal number of reference genes to be used in qPCR experiments was three (open circle).

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Molecular Pain

ISSN: 1744-8069

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