Table 1 Primer sequences for the candidate reference genes
Gene name | Accession number | Function | Sequence | Product size (base pairs) |
|---|---|---|---|---|
Hprt1 | Genbank: NM_012583 | Purine synthesis in salvage pathways | Forward 5′-CTCATGGACTGATTATGGACAGGAC Reverse 5′-GCAGGTCAGCAAAGAACTTATAGCC | 123 |
Actb | Genbank: NM_031144 | Cytoskeletal structural protein | Forward 5′-CCGCGAGTACAACCTTCTTG Reverse 5′-GCAGCGATATCGTCATCCAT | 81 |
Mapk6 | Genbank: NM_031622 | Member of the Ser/Thr protein kinase superfamily | Forward 5′-TAAAGCCATTGACATGTGGG Reverse 5′-TCGTGCACAACAGGGATAGA | 129 |
B2m | Genbank: NM_012512 | Beta-chain of major histocompatibility complex class I molecules | Forward 5′-CGAGACCGATGTATATGCTTGC Reverse 5′-GTCCAGATGATTCAGAGCTCCA | 114 |