Behavioural changes following application of the cuff. Results for the ipsilateral hindpaws are shown for both neuropathic (n = 10) and sham-operated (n = 10) Wistar rats while the contralateral values are shown for neuropathic rats only. Each point represents the mean ± SEM as a percentage of sham values (percentage of sham ipsilateral hindpaw for neuropathic ipsilateral results and percentage of sham contralateral hindpaw for neuropathic contralateral results). A) 50% withdrawal threshold to von Frey hairs in neuropathic and sham-operated rats as a percentage of sham values obtained with the up-down method. B) Assessment of thermal hyperalgesia using the Hargreaves’ test. Withdrawal threshold to light stimulus as a percentage of sham values. In A and B, comparison to sham ipsilateral hindpaw *P < 0.05, **P < 0.01 by 2 way ANOVA with Bonferroni correction. Comparison to contralateral hindpaw of cuff animals #P < 0.05, ##P < 0.01.