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Figure 3 | Molecular Pain

Figure 3

From: Spatial and temporal pattern of changes in the number of GAD65-immunoreactive inhibitory terminals in the rat superficial dorsal horn following peripheral nerve injury

Figure 3

Representative confocal microscopy images of IB4 staining and GAD65 immunoreactivity from the dorsal horn of sham (A-C) and neuropathic (D-F) rats ipsilateral to injury at 21, 42, 56 days after surgery. Capital letters and lowercase letters denote images taken with 20X and 63X objectives, respectively. Lowercase italic letters show representative images of quantified regions in both lamina I and lamina II, taken with a 63X objective, and a zoom factor of 2. IB4-labelling is shown in green and GAD65 in red. Note that for each time point, the image with the largest loss of IB4 staining was chosen for illustration and this IB4 lesion is shown by a yellow rectangle. The smaller regions used for quantification were taken from within these yellow rectangles. High magnification images show a clear lack of IB4+ terminals and an important loss of GAD65-IR in lamina II at 21 days (d and d) in neuropathic animals. By 42 days (e and e) after surgery, IB4-IR and GAD65-IR began to be restored in the lesion, however by 56 days (f and f) there were still considerably fewer IB4+ terminals present when compared to sham (c and c).

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