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Figure 4 | Molecular Pain

Figure 4

From: Spatial and temporal pattern of changes in the number of GAD65-immunoreactive inhibitory terminals in the rat superficial dorsal horn following peripheral nerve injury

Figure 4

Quantitative analysis of the number of boutons in the superficial dorsal horn of the spinal cord in sham and neuropathic rats at various times after surgery. Quantification of terminal density was performed within the region of loss of IB4 staining. The number of ipsilateral profiles is shown for neuropathic and sham rats, with contralateral values shown only for neuropathic animals. Each point represents the mean ± SEM as a percentage of sham values. A) Quantitative analysis of the density of IB4-labelled terminals in lamina II in sham and neuropathic rats. Neuropathic rats had a drastic reduction in the number of IB4+ terminals ipsilateral to injury with maximal loss occurring at 21 days followed by a gradual recovery with numbers remaining significantly different from sham at 42, 56 days. There were always significantly fewer IB4+ terminals ipsilateral vs. contralateral to injury. B) Quantitative analysis of GAD65-IR terminals in lamina II in sham and neuropathic rats. Neuropathic rats had a progressive reduction in the number of GAD65-IR terminals ipsilateral to injury with significantly fewer profiles than sham at 10 days, and a maximal loss at 21 days. Despite a partial recovery, GAD65-IR terminals remained significantly reduced at 56 days compared to sham, and at 10, 14, 21, and 56 days were significantly lower than contralateral values. C) Quantitative analysis of GAD65-IR terminals in lamina I in sham and neuropathic rats. Neuropathic rats had a small but progressive reduction in the number of GAD65-IR terminals ipsilateral to injury with values significantly different from sham at 10, 14 and 21 days and recovery by 42 days. There was a similar loss of GAD65-IR terminals ipsilateral and contralateral to injury. *P < 0.05, **P < 0.01 sham ipsilateral vs. neuropathic ipsilateral #P < 0.05 neuropathic ipsilateral vs. contralateral.

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