Figure 2
From: Epigenetic regulation of spinal cord gene expression controls Opioid-Induced Hyperalgesia
HDAC inhibitor treatment increases spinal cord BDNF and dynorphin protein levels after morphine treatment. SAHA treatment resulted in significantly increased expression of BDNF after 1 or 7 days since cessation of morphine treatment compared to vehicle treatment (A). Levels of dynorphin were similarly increased on day 5 in SAHA and vehicle groups and it was significantly up-regulated 7 days after morphine and SAHA treatment only (B). HDAC inhibition was carried out for the duration of escalating dose morphine administration. Error bars: SEM, n = 5-6/group; *p < 0.05, **p < 0.01, ***p < 0.001 for comparison with vehicle and ###p < 0.001 for comparison with morphine treatment. Data were analyzed by two-way analysis of variance (ANOVA) followed by Tukey’s post-hoc test for multiple comparisons within each timepoint.