Figure 3

Selective acute inhibition of BDNF and prodynorphin signaling attenuates opioid induced hyperalgesia. Effects of single dose ANA-12 (TrkB receptor antagonist, 1 mg/kg) or nor-BNI (KOR antagonist, 1 mg/kg) in the setting of OIH was examined. Selective antagonism of TrkB (A) receptor and KOR (B) produces a reversal of mechanical hypersensitivity, whether given once at day 5 (day 1 after completion of morphine protocol) or day 12 (day 7 after completion of morphine). However, acute ANA-12 but not nor-BNI administration reversed the sensitization present in the morphine + SAHA group. Error bars: SEM, n = 6/group, *p < 0.05, **p < 0.01 and ***p < 0.001 for comparison between treatments for each timepoint. Data for each timepoint were analyzed by one-way ANOVA followed by Sidak multiple comparisons tests.