Figure 7
From: Epigenetic regulation of spinal cord gene expression controls Opioid-Induced Hyperalgesia
Spinal application of low dose BDNF or dynorphin A produces hyperalgesia in previously morphine treated mice. Effects of single intrathecal dose of (A) saline (5uL), (B) BDNF 1 ng/5uL or (C) Dynorphin A 1 nmol (5uL) in mice 7 days after cessation of morphine/saline treatment OIH were examined. The drug doses selected fail to produce mechanical hypersensitivity in animals previously treated with saline. However, in the setting of resolved OIH both agents produce robust mechanical allodynia. Error bars: SEM, n = 6/group, **p < 0.01 and ***p < 0.001 for comparison between treatments for each timepoint. Data for each timepoint were analyzed by two-way ANOVA followed by Sidak multiple comparisons tests.