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Figure 4 | Molecular Pain

Figure 4

From: Activated spinal astrocytes are involved in the maintenance of chronic widespread mechanical hyperalgesia after cast immobilization

Figure 4

Intrathecal application of L-α-AA inhibits bilateral astrocyte activation in CPCP rats. (A) Low-magnification images of GFAP immunoreactivity in L4 spinal segments. Scale bar = 200 μm. L-α-AA (150 nmol) or vehicle (PBS) was injected intrathecally 5 weeks after cast removal, and the former clearly decreased bilateral GFAP immunoreactivity. (B) Schematic illustration of L4. The predefined square area including the superficial dorsal horn (lamina I-III) (a) and deep dorsal horn (IV–V) (b) designates the area of quantitative analysis of GFAP astrocyte immunoreactivity. (C) High-magnification images of GFAP immunoreactivity in L4 lamina I–III. Scale bar = 50 μm. (D) High-magnification images of GFAP immunoreactivity in L4 IV–V. Scale bar = 50 μm. (E) The total area of GFAP immunoreactivity in L4 lamina I–III (n = 5). (F) Total area of GFAP immunoreactivity in L4 lamina IV–V (n = 5–6). Values are the mean ± SEM. *p < 0.05 as compared with the intact (without cast immobilization) group (unpaired t-test).

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Molecular Pain

ISSN: 1744-8069

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