Spatial distribution of network synaptic transmission in the ACC slice on a multi-channel array probe. (A) Schematic diagram of location of an ACC slice on the MED-64 probe (left) and the scale of the electrodes (right). (B) Light microscopy photograph showing the relative location of ACC slice and MED-64 probe. The yellow circle is the selected stimulated site. (C-D) Two mapped figures showed the evoked potentials in all active channels in 1 slice 0.5 h before (black) and 3 h after TBS (yellow lightning bolt). Channels with L-LTP and no L-LTP were marked as “a” (green) and “b” (blue), and the TBS-recruited synaptic responses were marked as asterisks, respectively. Vertical lines indicated the layers of ACC slices. (E) The sample traces and the plotted slope results of one channel showing L-LTP (Ch. 35) and the other without showing L-LTP (Ch. 34) of the fEPSP from one slice in the superficial layer. (F) The plotted slope showed the L-LTP of all 6 channels in the superficial layer. (G) The sample traces and the plotted slope results of one channel showing L-LTP (Ch. 36) and the other without showing L-LTP (Ch. 21) of the fEPSP from the same slice in the deep layer. (H) The plotted slope showed the L-LTP of all 12 channels in the deep layer. (I) The summarized L-LTP of the fEPSP slopes of 6 slices/6 mice. (J) The summarized fEPSP slopes of 6 slices/6 mice showed that, if no TBS induction was applied, the basal synaptic transmission kept stable.