Figure 4

Rats with AHF chronic pancreatitis developed secondary hypersensitivity. A. Hindpaw Mechanical Allodynia. The baseline for the 50% paw withdrawal threshold to mechanical stimulation (von Frey fiber) was similar in control chow fed rats and the AHF fed group. The mechanical threshold was progressively decreased in the AHF fed group starting from week 3 and reaching maximal hypersensitivity at week 5. The paw mechanical allodynia remained through the entire experiment time course (Two-way ANOVA, Bonferroni post-test,***p < 0.001). B. Abdominal Secondary Heat Hyperalgesia. The baseline for abdominal withdrawal latency (ABWL) was similar between control chow and AHF fed groups. Secondary heat hyperalgesia developed on the abdominal skin area in the fourth week of AHF feeding. The abdominal withdrawal latency (ABWL) baseline of 13.39±1.4 s was decreased to 8.77±0.28 s and remained at this level throughout the experimental time course. This was a statistically significant difference compared to the control chow fed group (Two-way ANOVA, Bonferroni post-test, ***p < 0.001). C. and D. 44°C Hotplate Hypersensitivity Assay. C. The nocifensive responses were recorded for 10 min (1 minute per bin). The event/time curve in rats with pancreatitis showed a statistically significant left-ward shift compared to that of the normal chow fed control group (Two-way ANOVA, Bonferroni post-test, n = 6/group, *p < 0.05). D. The rearing events on the hotplate were significantly increased for rats with AHF pancreatitis compared to normal chow fed rats (t-test, *p < 0.05).