Figure 3

The effect of intrathecal 100 μM SB366791 treatment on the responsiveness to mechanical stimuli after plantar incision. The number of responses to a range of von Frey filaments applied to the plantar skin of both the incised and the contralateral intact hindpaws, before and after the surgical procedure, was measured with the same pattern and testing frequency. Testing the incised skin after the intrathecal injection of vehicle (15 min before the surgery) and the corresponding contralateral intact hindpaw (n = 6) indicated the development of postoperative mechanical allodynia (10, 19, 36, 59, 80 mN) and hyperalgesia (144, 292, 367 mN). Intrathecal injection of 100 μM SB366791 (15 min before the surgery) showed a moderate antinociceptive effect of the TRPV1 receptor antagonist on postoperative mechanical allodynia, while mechanical hyperalgesia remained unaffected (n = 6). *P <0.05, **P <0.01, SB366791 injection: incision versus contralateral side; ⁺P <0.05, ⁺⁺P <0.01, vehicle injection: incision versus contralateral side; ^#P <0.05, SB366791 versus vehicle injection on the incised hind paw. The time of drug administration is mark by arrow.