Figure 3

CUR and SAHA differently affect the analgesic efficacy of LY379268 in the mouse formalin test. (A, B) CUR-treated mice (100 mg/kg, ip for three consecutive days) did not significantly differ from vehicle-treated mice. The acute administration of LY379268 (3 mg/kg, i.p.) 30 minutes before formalin injection significantly reduced both phases in mice. A single administration of LY379268 (3 mg/kg, i.p.) 30 min before formalin in CUR-pretreated mice failed to induce analgesia in both phases of the formalin test. (C, D) SAHA treated mice (5 mg/kg, sc, for 5 consecutive days) significantly reduced the licking behavior in the second phase of the formalin test. A single administration of LY379268 (3 mg/kg, i.p.) 30 minutes before formalin injection significantly reduced both phases in mice. The analgesic effect of LY378268 acutely injected 30 min before formalin was potentiated in SAHA-pretreated mice. Data represent the mean ± S.E.M. of 12 to 16 mice per group. *p < 0.05 (Two-way ANOVA + Bonferroni) versus the respective vehicle group, °p < 0.05 (Two-way ANOVA + Bonferroni) versus the corresponding group treated with SAHA.