Ablation of CGRPα sensory neurons reduces the number of NF200-expressing DRG neurons. (A-F) Lumbar DRG from Cgrpα-DTR+/- mice treated with saline or DTX were stained with antibodies to CGRP (A and D, red), NF200 (B and E, green), and NeuN (A-F, blue). (C and F) Merged images. Images were acquired by confocal microscopy. Scale bar in F is 100 μm. (G) Quantification of the number of cells stained for CGRP and NF200. Cell counts are representative of n = 3 mice per treatment group, mean ± SEM. *p <0.05, **p <0.005, ***p <0.0005. Tissue was collected 7 days after the second saline/DTX injection.