Ablation of CGRPα sensory neurons in DTR-Trpm8-/-
mice reduces heat sensitivity without affecting mechanical sensitivity. Saline- and DTX-treated Cgrpα-DTR+/- and DTR-Trpm8-/- mice were tested in the (A) tail immersion assay at 46.5°C, (B) tail immersion assay at 49°C, (C) 52°C hot plate assay, (D) Hargreave’s assay (radiant heating of the hindpaw), and (E) electronic von Frey assay (to measure mechanical sensitivity). (F) Innocuous mechanical sensitivity was measured using the cotton swab assay. All values are represented as mean ± SEM. t-tests were used to compare time points between pre-saline/DTX and post-saline/DTX-treated mice. *p < 0.05, **p < 0.005, ***p < 0005.