Ablation of CGRPα-expressing DRG neurons had similar effects on body weight and thermoregulation in Cgrpα- DTR+/-
mice. (A and B) Body weight and (C and D) rectal temperatures of Cgrpα-DTR+/- and DTR-Trpm8-/- mice before and after saline/DTX treatment. (E) The water repulsion was performed 7 days after saline/DTX injection. Mice were immersed in a 37°C water bath for 2 min. Body weight was measured every 5 min., starting before immersion and recorded every 5 min afterwards for 60 min. All values are represented as mean ± SEM. t-tests were used to compare time points between pre-saline/DTX and post-saline/DTX-treated mice. *p < 0.05, **p < 0.005, ***p < 0005. Asterisks colors for DTX-treated experimental groups are relative to saline-control groups.