Figure 3
From: Optogenetic activation of brainstem serotonergic neurons induces persistent pain sensitization
Optical stimulation increases c-fos expression of RVM 5-HT neurons selectively in TPH2-ChR2 mice. (A) Representative images showing anti-c-fos, anti-TPH2 and merged staining after single light stimulation in the RVM of WT mice (top row) and TPH2-ChR2 mice (bottom row). Scale bars =100 μM. (B and C) Summarized data of intensity of c-fos staining (B) and percentage of c-fos-positive cells vs. DAPI-positive cells (C) in the stimulated RVM of WT and TPH2-ChR2 mice (9 slices from 3 different mice for each group). *** p <0.001 (unpaired t test).