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Figure 2 | Molecular Pain

Figure 2

From: Sphingosine 1-phosphate to p38 signaling via S1P1 receptor and Gαi/o evokes augmentation of capsaicin-induced ionic currents in mouse sensory neurons

Figure 2

Inhibition of downstream signaling mediated by S1P largely prevents potentiation of I CAPS in sensory neurons. A, inhibition of heteromeric G-proteins by inclusion of 3.0 mM GDPβS in the patch pipette fully prevented the potentiation of ICAPS mediated by 1.0 μM S1P. Furthermore, inhibition of Gαi by pertussis toxin (100 ng/ml PTX, B), PI3Kγ (100nM AS605240, C) and PKC (1.0 μM BIM-1, D) decreased the amplitude of S1P mediated ICAPS potentiation in sensory nociceptors significantly compared to the enhanced ICAPS after S1P stimulation (E). ***p < 0.001, **p < 0.01, *p < 0.05, MWU-test, numbers within the bars represent the number of individual cells recorded.

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