Table 1 Sequences of primers used for RT-PCR
Target name | Forward sequence | Reverse sequence |
|---|---|---|
Gapdh | TCA ACT ACA TGG TCT ACA TGT TCC AG | TCC CAT TCT CAG CCT TGA CTG |
Hprt | CTC ATG GAC TGA TTA TGG ACA | GCA GGT CAG CAA AGA ACT TAT |
SP | TGG CGG TCT TTT TTC TCG TT | GCA TTG CCT CCT TGA TTT GG |
CGRP | GTG TCA CTG CCC AGA AGA GAT C | CAA AGT TGT CCT TCA CCA CAC C |
IL-1β | GAAAGACGGCACACCCACC | AAACCGCTTTTCCATCTTCTTCT |
Dcir1 | CCATAGCAAGGAAGAACAGGACTT | TGAATCCCAGAGCCCTATAAAATAA |
Dcir2 | CCATCATCCAAGTAAGCCAGGTTC | GAGTCAGTTGAAGTAAAGTAGCAGTAG |
Dcir3 | TGCCACAAGTTCTCCAAG | TCCAATTCAGTATAGTTCAGTTCC |
Dcir4 | CATTCGTCCGTGGAAGACAAA | TGCAGAGTCCCTGGAAGTGAA |
Dcar1 | TGCTCATCTGTTGGTGATCCA | TGTAAAATAACCCCAACGAGTGTCTA |
Mcl | CACAAGGCTAACATGCATCCTAGA | GCAAAGTAACAGTTAGACTGGAATGCT |
Mincle | TTTCACAGAGTCCCTGAGCTTCT | TCCCTCATGGTGGCACAGT |
TNF-α | GACCCTCACACTCAGATCCAGATCATCTTCT | ACGCTGGCTCAGCCACTC |
Ccr-2 | AGAAGTATCCAAGAGCTTGATGAGG | ATAGTGAGCCCAGAATGGGAG |
Ccl-2 | AACTCTCACTGAAGCCAGAT | GGTGACAAATACTACAGCTTC |
Cx3cr1 | CTGCTCAGGACCTCACCAT | CAGACCGAACGTGAAGACAA |
Cathepsin S | TGT TCT CGT GGT TGG CTA T | AAC GGT TTA GAT TTC TGG GT |
IL-6 | AGAAAAGAGTTGTGCAATGG | ACAAACTCCAGGTAGAAACG |