Figure 4
The effect of pre-incubating the GRP antibody with GRP and NMB. a, Immunostaining with the GRP antibody in the superficial dorsal horn from a transverse section. b, An equivalent field from a section in which the GRP antibody had been preincubated with GRP at 10−7 M overnight. c, Staining with antibody that had been preincubated with NMB at 10−5 M overnight. Note that preincubation with GRP completely blocked specific staining, whereas the staining was not affected by NMB. Scale bar = 20 μm.