Figure 8

Lack of Pax2 in EGFP cells in the GRP-EGFP mouse. a-c, Immunostaining with antibodies against EGFP (green), Pax2 (blue) and NeuN (red) in a single optical section. d shows a merged image. Several EGFP⁺ cells, all of which are Pax2⁻ are visible, and 3 of these are marked with arrows. Note the very weak NeuN staining in these cells, compared to that seen in other nearby neurons, including those that are Pax2⁺, some of which are indicated with arrowheads. Scale bar = 50 μm.