Figure 2

Lentiviral transduction of neuroblastoma cell line. Lentiviruses encoding SHG or Endomorphins were used to transduce a neuroblastoma cell line (SH-SY5Y). 5 days post-transduction cells were fixed and stained with anti-SHG or anti-endomorphins (1 and 2) antibodies. An image of negative control shows the absence of fluorescence (A). The culture was counter stained DAPI (blue) to identify nuclei (B). Green fluorescence in (C) and red fluorescence in (D) correspond to SHG and Endomorphin 1 or 2 respectively. Micron bar = 50 μm.