Effect of chloroquine intratechal injection on Beclin 1, LC3 and p62 in the spinal dorsal horn. (A) Representative Western blots of Beclin 1, LC3 and p62 protein levels after chloroquine (300pmoles/3 μl, i.t.) according to the treatment protocol in Figure 6A. (B) Densitometric analysis of Beclin 1, LC3-II and p62 expression. While Beclin 1 expression was not affected, LC3-I, LC3-II and p62 levels were significantly increased in the chloroquine group compared to the vehicle-injected group. The appearance of a second band has been previously suggested as a p62 splicing variant or a partially cleaved product . Signals of each band were normalised to the respective GAPDH. Data were expressed as mean ± SEM. *P < 0.05. Vehicle n = 3, choloroquine (CQ) n = 6 (n = 5 for Beclin 1).