Figure 2

Whole-cell recordings in the current-clamp modus. A) A neuron which was in carbogen-bubbled control solution (pO₂ 600 mmHg, left) not spontaneous active, responded in hypoxic solution (pO₂ 27 mmHg, middle) spontaneously (1 pA). After reoxygenation the effect was reversible and the neurones did not generate an AP. B) The same neuron as shown in A. The neuron generates a second AP in the hypoxic solution compared to control (middle vs. left) but the AP-amplitude was reduced from 99 mV to 79 mV and the duration of the AP was prolonged in hypoxic solution from 2.3 ms to 2.6 ms (measured at half amplitude). C) Same neuron as shown in A and B. Trains of APs elicited in a DRG-neuron by a long lasting depolarizing current injection (750 ms, left). After reduction of pO₂ (27 mmHg, middle) the neuron generate few APs and adapted. After reoxygenation the effect was reversible (right). 23°C; Pipette solution: High-Ki EGTA; Bath: Ringer's solution.