Figure 3
From: Enhanced group II mGluR-mediated inhibition of pain-related synaptic plasticity in the amygdala
Miniature EPSC (mEPSC) analysis indicates pre- rather than post-synaptic effects of LY354740. A: Original current traces of mEPSC recorded in an individual CeLC neuron in the presence of TTX (1 μM) show that LY354740 (1 μM) reduces frequency but not amplitude of mEPSCs. The CeLC neuron was recorded in a slice from an arthritic rat. B, C: Normalized cumulative distribution analysis of mEPSC amplitude and frequency shows that LY354740 causes a significant shift toward higher inter-event intervals (decreased frequency) (B, P < 0.005, maximal difference in cumulative fraction = 0.183, Kolmogorov-Smirnov test) but had no effect on the amplitude distribution (C). LY354740 selectively decreased mean mEPSC frequency (events/s) (P < 0.05, paired t-test) but not mEPSC amplitude in the sample of neurons (n = 4; see bar histograms in B, C). Symbols and error bars represent mean ± SE. Neurons were recorded in voltage-clamp at -60 mV. * P < 0.05.