Figure 4

Activation of group II mGluRs has no significant effect on current-voltage relationship and membrane slope conductance. A: Voltage-clamp recordings of whole-cell currents elicited in one CeLC neuron by a series of 400 ms voltage steps (-110 to -40 mV) from a holding potential of -60 mV in control ACSF and during application of LY354740 (1 μM, 10 min). B: Current-voltage relationship of the same neuron as in A was calculated from the steady state current traces in A. C: Membrane slope conductance (in nS) is calculated from the current-voltage (I-V) relationships constructed from steady-state current traces like those shown in A in the presence and absence of the different concentrations of LY354740. In the sample of CeLC neurons (n = 13) LY354740 (1–1000 nM) had no significant effect on membrane slope conductance compared to predrug control "0" (P > 0.05, ANOVA with post-hoc Dunnett's test). Symbols and error bars represent mean ± SE. Neurons were held at -60 mV.