Figure 4From: Impaired inflammatory pain and thermal hyperalgesia in mice expressing neuron-specific dominant negative mitogen activated protein kinase kinase (MEK)Basal phosphorylation of ERK is similar in the wild type and DN MEK mice spinal cords. A, Representative immunoblot of mouse spinal cord homogenates using a phospho-ERK1/2 antibody (top) or a total ERK1/2 antibody (bottom). The arrows show the position of the 44 kDa ERK 1 and 42 kDa ERK 2 isoforms. Quantification of ERK activation in male (B) and female (C) mice. Phospho-ERK bands were densitized and normalized to total ERK from the same samples. n = 8–9 (male mice), and 8–9 (female mice).Back to article page