Figure 4

Representative images showing Nav1.1 (A, B), Nav1.2 (C, D), and Nav1.6 (E, F) mRNA transcripts within regions corresponding to the ipsilateral (A, C, E) and contralateral (B, D, F) VPL in coronal brain sections collected at bregma -3.14 10 days following CCI. Signal was detectable in cells exhibiting a neuronal morphology. Quantification of the number of neurons per section expressing each sodium channel isoforms (G) did not reveal any significant differences in ipsilateral or contralateral expression after CCI when compared to intact animals. Quantification of sodium channel in situ signal intensity (H) showed moderate expression of all channel isoforms in intact animals, and that 10 days after CCI no isoforms exhibited changes in signal intensity.