Figure 2

(A-D) RT-PCR analysis of changes in NMDA NR1splice variants in rat descending colon following TNBS induced colitis. Examples of agarose gel electrophoresis of PCR products are shown on the top of each panel. The RT-PCR products with specific size are indicated. The samples from naïve animals are shown in "N", saline control animals are shown in "S". GAPDH PCR was used as an internal Control. The bottom of each panel shows the summary of the effects on NMDA NR1 receptor splice variant mRNA expression. Each value represents mean of three Individual experiments. The asterisks indicated significant differences from respective controls (One ANOVA with Dunnett's post-test comparison test); p < 0.05. Statistical comparisons were made among all groups using raw data. The mRNA expression of NR1 subunit normalized to GAPDH expression are present as the Means ± SEM. A. Expression of NR1 pan mRNA increase at day 14, 21 and 28 following colitis B. Expression of NR1 C1 cassette mRNA appeared at day 14, 21 and 28 after colitis C. Expression of NR1 N1 cassette of N1 plus mRNA appeared at day 14, 21 and 28 days after colitis; expression of NR1 N1 minus was not regulated by TNBS induced colitis D. Expression of NR1 C2 cassette mRNA was not regulated by TNBS induced colitis