Figure 3

Acute and inflammatory pain behaviour in Na _v 1.3 KO is normal. Thermal threshold in the Hargreave's (A, WT 7.83 ± 0.7 sec, n = 8; KO 7.82 ± 0.6 sec, n = 11; P = 0.9) and hotplate apparatus (B, at 50°C WT 41.3 ± 7.3, n = 13; KO 44.2 ± 6.5, n = 13; P = 0.7. at 55°C WT 16.6 ± 2.4; KO 14.3 ± 1.8; P = 0.1) were similar. Mechanical threshold to range of von Frey hairs applied to hindpaw (C) and plunt force applied to tail (D, WT 157 ± 22.7 g, n = 13; KO 145 ± 18.2, n = 13; P = 0.4) were not different. Both phase of the formalin pain response were not different (E, phase 1 WT 86.1 ± 12.9 sec, n = 8; KO 78.4 ± 9.2, n = 8; P = 0.6. Phase 2 WT 210 ± 38.3 sec; KO 227.4 ± 24.2; P = 0.7). Injection of CFA in hindpaw elicited thermal (F) and mechanical hyperalgesia 2 days post injection (G, WT 0.3 ± 0.08 gram, n = 6; KO 0.23 ± 0.05, n = 6; P = 0.5) to the same level in both mice groups.