Figure 4
From: HSV-mediated expression of interleukin-4 in dorsal root ganglion neurons reduces neuropathic pain
IL-1β, PGE2, and p-p38 expression in the spinal cord dorsal horn in neuropathic rats. Rats were given gentle tactile stimulation to left paw for 10 min 3 weeks after SNL or sham surgery. Ipsilateral dorsal horn was harvested 2 h after gentle stimulation. Inoculation of S4IL4 but not control vector significantly suppressed the increase in levels of IL-1β (a), PGE2 (b), and p-p38α (c) (all values in pg/mg wet weight of tissue; *P < 0.05, **P < 0.01, ANOVA, post-hoc Scheffe test). (d) Double immunofluorescence demonstrates colocalization of p-p38 (red) with the microglial marker OX-42 (green) in dorsal horn 3 weeks after SNL. P-p38 did not colocalize with NeuN in spinal dorsal horn (data not shown).