Pharmacology of temperature effect on net excitation. A) Antagonism by CPZ (20 μM) and a Ca++-free medium. Upper inserts show the time traces of optical signals at 27°C (thin) and 33°C (bold). Bottom graph shows the averaged changes in normalized magnitude of optical signal obtained in 4 slices when temperature was altered from 27°C to 33°C as indicated by the bar above. Circles, squares and diamonds indicate results obtained in the normal solution (control), in a Ca++-free medium, and CPZ-containing solution, respectively. B) Antagonism by suramin (100 μM) and potentiation by TNP-ATP (1 μM). Bottom line in the graph indicates the duration of drug application. C) No effect of BMI (10 μM) and STRY (3 μM) (squares), and Ni++ (diamonds) is observed on temperature effect. D) Augmented effect in APV (50 μM) and CNQX (10 μM).