Quantitative downregulation of mature miRNAs in the TG V3 following unilateral CFA injection. Total RNA was extracted by the Absolutely RNA kit (Stratagene, Lo Jolla, CA) and 10 ng of RNA was used to generate cDNA using a TaqMan miRNA RT kit and TaqMan miRNA primers specific to mature miRNA (ABI). Each TaqMan PCR reaction contained cDNA derived from 0.88 ng RNA. Changes in miRNA level were calculated by a ΔΔCt method  and are presented as percentage of control (the basal level in naïve animal) in mean + s.e. from 6 to 12 samples. Each sample was measured in triplicate. Sample differences were examined by one-way ANOVA separately for each miRNA. *: P < 0.05 when compared to the control.