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Figure 3 | Molecular Pain

Figure 3

From: Null effect of antidepressants on the astrocytes-mediated proliferation of hippocampal progenitor cells in vitro

Figure 3

Effects of antidepressant-treated astrocytes on the proliferation of NPCs in a contact-dependent culture system. (A) Proliferation of NPCs co-cultured with astrocytes using an overlay co-culture system. After treating astrocytes with antidepressants, NPCs were overlaid on the astrocytes. NPCs were labeled with GFP using a retrovirus. After BrdU immunocytochemical analysis, the nuclei were stained with DAPI (blue). An arrowhead (pink) indicates BrdU and GFP double-positive NPCs, and the arrow (pink) shows BrdU-positive cells. These BrdU-only-positive cells represent astrocytes or NPCs that were not infected with the GFP-expressing retrovirus. Scale bar, 50 μm. (B) Quantification of the proliferation of NPCs. BrdU and GFP double-positive cells were counted. In comparison with naïve astrocytes, those treated with 10 μM of desipramine or fluoxetine did not further increase the proliferation of NPCs. Data shown are mean values ± SEM. One-way ANOVA and Tukey's multiple comparison test were used for data analysis.

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