Figure 1
From: Spinal NKCC1 blockade inhibits TRPV1-dependent referred allodynia
NKCC1 inhibitor bumetanide blocks intracolonic capsaicin-evoked referred allodynia and hyperalgesia. Three doses of bumetanide or vehicle (VEH) were injected intrathecally (IT) and saline or capsaicin was injected into the colon 5 min later. A and B) Baseline response frequencies to abdominal 1 mN and 32 mN von Frey hair application are shown as well as 30 min after the intracolonic (IC) saline injection. C and D) Response frequencies to abdominal 1 mN (C) and 32 mN (D) von Frey hair application 30 min after the IC capsaicin injection are shown in comparison to baseline von Frey response frequencies. Stars (*) indicate significant effects vs. baseline (two-way anova); number signs (#) indicate significant effects of IT bumetanide injections (one-way anova, # or * p < 0.05, ** p < 0.01, *** p < 0.001; n = 6 per group).