Figure 5

NADA-evoked stroking allodynia is TRPV1-dependent and inhibited by bumetanide. The endogenous TRPV1 agonist NADA (10 nmol) or vehicle (VEH) was injected intrathecally (IT) with or without a co-injection of the TRPV1 antagonist AMG 9810 (1 nmol) and the hindpaws were stimulated with a cotton bud 15 (A) and 60 min (B) after the IT injection. NADA (10 nmol) or VEH was injected IT with or without a co-injection of the NKCC1 antagonist bumetanide (BUM, 1 nmol) and the hindpaws were stimulated with a cotton bud 15 (C) and 60 min (D) after the IT injection. Response frequencies to the cotton bud stimulation are shown. Stars (*) above columns indicate significant effects of NADA vs. VEH while number signs (#) above horizontal bars indicate significant effects of antagonists (one-way anova, * or # p < 0.05, ** or ## p < 0.01; n = 6 per group).