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Figure 6 | Molecular Pain

Figure 6

From: Prostaglandin E2 potentiation of P2X3 receptor mediated currents in dorsal root ganglion neurons

Figure 6

PGE2 sensitizes the mechanical allodynia and thermal hyperalgesia produced by α,β-meATP. (A) Mechanical allodynia. Paw withdrawal threshold was measured 10 min after paw injection of α,β-meATP (1 nmol/50 μl) or PGE2 ((0.05 nmol/50 μl). The data were normalized with baseline responses before either injection. α,β-meATP and PGE2 applied individually resulted in a moderate decrease in the threshold [(1-meATP/Con) = 0.22 ± 0.04; (1-PGE/Con) = 0.16 ± 0.03, n = 3)]. However, co-injection of α,β-meATP and PGE2 produced a much larger decrease in the threshold [1-(meATP+PGE)/Con = 0.65 ± 0.07, n = 3] than adding the threshold reduction produced by α,β-meATP and by PGE2. H89 (0.5 nmol/50 μl) reversed the enhanced allodynia produced by PGE2. (B) Thermal hyperalgesia. α,β-meATP and PGE2, applied separately, produced a small reduction in the paw withdrawal latency [(1-meATP/Con) = 0.08 ± 0.04; (1-PGE/Con) = 0.18 ± 0.02, n = 3]. Co-injection of PGE2 and α,β-meATP produced a much larger reduction in the paw withdrawal latency [(1-(meATP+PGE)/Con = 0.54 ± 0.04, n = 3]. H89 blocked the enhancing effect of PGE2. (* P < 0.05, Two-way ANOVA).

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