Figure 7
From: Morphine reduces local cytokine expression and neutrophil infiltration after incision
Confocal neutrophil-cytokine double labeling experiments using incised skin. For these studies skin from the wound edges was harvested 2 hours after incision. After sectioning, the tissue was exposed to anti-neutrophil and anti-IL-1β antibodies followed by the application of CY3 (green fluorescence, neutrophils, panel A) and FITC (red, IL-1β, panel B) conjugated secondary antibodies. Panel C presents the merged image with arrows pointing to several strongly double labeling cells. The scale bar in panel C is 50 μm in length. These micrographs were taken of dermal tissue under 630× magnification.