Cooling decreases current density for Nav1.7 and L858F. A. Representative current-voltage (I-V) families recorded from HEK293 cells stably expressing Nav1.7 (left column) or the mutation L858F (right column) at 16°C, 25°C or 35°C. Cells were held at -120 mV and depolarizing steps were applied to membrane potentials ranging from -80 mV to 40 mV in 5 mV steps. B. Temperature dependence of the current density for Nav1.7 (black bars, n = 17, 15, 25) and L858F (white bars, n = 15, 16, 28) at the indicated temperatures. Current density was measured as peak current divided by cell capacitance. * indicate significant differences between values with p < 0.05, tested with ANOVA and Tukey HSD post hoc analysis.