Activation midpoint shifts to more depolarized potentials upon cooling for L858F, but not for Nav1.7. A. Voltage dependences of conductance for Nav1.7 at 16°C (filled squares, n = 12), 25°C (filled triangles, n = 13) and 35°C (filled circles, n = 12). Conductance curves were derived from current-voltage families, normalized, and fitted with a Boltzmann equation, as described in methods. B. Voltage dependences of conductance for L858F at 16°C (open squares, n = 8), 25°C (open triangles, n = 15) and 35°C (open circles, n = 14). C. Midpoint of activation for Nav1.7 (black bars) and L858F (white bars) plotted versus temperature. D. Slope factor of activation for Nav1.7 (black bars) and L858F (white bars) plotted versus temperature. * indicate significant differences between values with p < 0.05, tested with ANOVA and Tukey HSD post hoc analysis.