Cooling increases currents elicited by slow ramp depolarizations, and diminishes the difference between Nav1.7 and L858F. Representative current traces of Nav1.7 (A.) and L858F (B.) ramp currents at 16°C, 25°C and 35°C. Cells were held at -120 mV and stimulated with a depolarizing voltage ramp that increased to 20 mV within 600 ms. C. The bar graph shows the mean peak currents recorded during the voltage ramps expressed as percent of transient peak current obtained during initial I-V families, 3 min after breaking into the cell; black bars represent Nav1.7 at 16°C (n = 8), 25°C (n = 9) and 35°C (n = 7); white bars represent L858F at 16°C (n = 8), 25°C (n = 9) and 35°C (n = 8). * indicate significant differences between values with p < 0.05, tested with ANOVA and Tukey HSD post hoc analysis.