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Figure 3 | Molecular Pain

Figure 3

From: Neurokinin-1 (NK-1) receptor and brain-derived neurotrophic factor (BDNF) gene expression is differentially modulated in the rat spinal dorsal horn and hippocampus during inflammatory pain

Figure 3

Representative images of Western blot analysis showing the effects of CFA-induced hyperalgesia on p-ERK1/2 and p-CREB protein levels in the rat hippocampus vs. the ipsilateral dorsal horn of the spinal cord. Total protein from nuclear fractions of the tissue was immunoblotted with either monoclonal anti-phosphoERK1/2 (p-ERK1: Mr = 44 kDa; p-ERK2: Mr = 42 kDa) or monoclonal anti-phosphoCREB (p-CREB: Mr = 43 kDa) antibody. Tissue levels of constitutively expressed total ERK and total CREB proteins were used as loading controls. Proteins were visualized using secondary antibodies conjugated to HRP and a chemilluminescence detection system.

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Molecular Pain

ISSN: 1744-8069

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