Figure 2
From: Endothelin potentiates TRPV1 via ETA receptor-mediated activation of protein kinase C
Potentiation of capsaicin-activated currents in dorsal root ganglion neurons by ET-1. A, Recording of current at -60 mV showing responses to repetitive applications of capsaicin (10 nM) in a Ca2+-free extracellular solution. Application of ET-1 (100 nM) at the time indicated by the bar resulted in a small increase in current during the ET-1 application and a large potentiation of the capsaicin-activated currents. B, Results of a similar experiment to that in A which shows a larger current response to ET-1 application. C, Bar graph summarising the potentiation of capsaicin responses by ET-1 (100 nM) in ET-1-responsive rat DRG neurons. Control is the normalized capsaicin response before ET-1 application, after ET-1 the first (n = 9, p = 0.0066) and second (n = 8, p = 0.017) normalized responses after ET-1 application.