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Figure 1 | Molecular Pain

Figure 1

From: Requirement of extracellular signal-regulated kinase/mitogen-activated protein kinase for long-term potentiation in adult mouse anterior cingulate cortex

Figure 1

Postsynaptic inhibition of PD98059 prevents the induction of cingulate LTP. A: A scheme illustrating the LTP induction protocol consisting of pairing 80 presynaptic pulses at 2 Hz with postsynaptic depolarization (holding at + 30 mV). B: LTP is induced by the pairing protocol in cingulate pyramidal neurons (n = 13). C: The MEK inhibitor, PD98059 (50 μM, n = 10) in the intracellular solution completely blocks LTP induction. Traces show averages of six EPSCs at baseline response (pre) and 30 min (post) after the pairing protocol (arrow). The dashed line indicates the mean basal synaptic response. D: Summary of the effects of PD98059 at different concentrations on LTP induced by the pairing protocol (PD98059; 5 μM, n = 8; 15 μM, n = 6, 50 μM, n = 10). * P < 0.05 compared to baseline response. E: The MEK inhibitor U0126 (50 μM) in the intracellular solution completely blocks LTP induction. Traces show averages of six EPSCs at baseline response (pre) and 30 min (post) after the pairing protocol (arrow). The dashed line indicates the mean basal synaptic response. F: Summary of the effects of MAPK inhibitors on LTP induced by the pairing protocol (U0126; n = 7, SP600125; n = 6, SB203850; n = 6). * P < 0.05 compared to baseline response.

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