Mean threshold force required for paw withdrawal to Von Frey stimulation at 1, 3, 7, 14, 21, 28, 35 and 42 days following LPC-induced focal nerve demyelination. Each data point is the mean threshold (± SE) force on the hindpaw ipsilateral (black circle) or contralateral (white circle) to the focal nerve injury site eliciting a withdrawal response (n = 10). Reduced behavioral thresholds for the hindpaw ipsilateral to the nerve lesion were significantly different from pre-operative baseline on postoperative days 1–28. The threshold force for the hindpaw contralateral to the nerve lesion did not reach significance until postoperative day 3, and significant differences were observed until postoperative day 28. The time course of sham injury (n = 6) is also represented but did not differ from the uninjured animals. Analysis was performed using two-way ANOVA followed by the Bonferroni post-hoc pair-wise comparisons (*p < 0.01).B) LPC-induced focal nerve demyelination did not produce changes in thermal responses as assessed by the Hargreaves test. Each bar is the mean withdrawal latency (± SE) of the hindpaw ipsilateral (white bar) or contralateral (black bar) to the focal nerve demyelination injury at postoperative day 7 and 14 (n = 10).