Expression of CXCR4 mRNA in rat lumbar DRG ipsilateral to focal nerve demyelination. Low (A) and high power (C) photomicrographs of CXCR4 mRNA transcripts present in lumbar DRG removed from vehicle-treated rodents at POD14 (n = 3). Many non-neuronal cells strongly expressed CXCR4. (C) Some presumptive neurons expressed low levels of CXCR4 mRNA (white arrow). Low (B) and high power (D) photomicrographs of CXCR4 mRNA transcripts present in lumbar DRGs derived from injured rats at POD14 (n = 4). The expression level and number of non-neuronal cells exhibiting CXCR4 mRNA transcripts in the lumbar DRG did not change following focal nerve demyelination. However, many neurons upregulated CXCR4 mRNA expression (D; white arrows indicate neurons with low levels of mRNA transcripts; white arrowhead points to a neuron lacking CXCR4 mRNA expression). (Scale bar is 1 mm (A and B); 40 μm (C and D).