Colocalization of IP-10 immunoreactivity (-ir) and isolectin B4 (IB4)-binding neurons in the lumbar DRG of naïve rat and rats subjected to LPC-induced nerve injury. IB4-binding in rat DRG neurons distinguishes a population of C-fiber nociceptors. A) The majority of IP-10-ir cells were limited to medium diameter neurons in the lumbar DRG from vehicle-treated rats (red arrows). D) IB4-binding small diameter presumptive nociceptors (green arrows) did not colocalize with IP-10-ir lumbar DRG neurons from vehicle-treated rodents at POD7 (G, merged images). B) Lumbar DRG ipsilateral to focal nerve demyelination exhibited numerous medium and small diameter IP-10-ir neurons at POD7. Limited numbers of neurons were positive for both IP-10-ir (B, red arrows) and IB4-binding (E, green arrows) on POD7 (H, merged images). C) Many IP-10-ir neurons (red arrows) colocalized with IB4-binding neurons (F, green arrows) at POD14 (I, merged images). Yellow arrows indicate colocalized cells. Scale bar is 100 μm.